Avaliação de alterações imunológicas provocadas pela obesidade induzida por dieta hipercalórica em camundongos C57BL/6 infectados por Leishmania chagasi

Abstract

Environmental factors, including diet, play a central role in the balance of normal immune homeostasis, is aldeady know that diets rich in simple carbohidrate or in saturated fat acids can influence the secretion of immune mediators altering the metabolism. However, many of the cellular mechanisms that control this maintainense or disturbence of this balance remain to be elucidated. Together with this, there is no results showing the impact of a hypercaloc diet on intracellular parasite response. Using mouse model feeding with high sugar and high fat diet, we examined the influence of this diet on the visceral leishmaniasis development caused by L. chagasi. C57BL/6 female mice (6-8 weeks) fed with control (AIN93G) or high sugar and fat diet (HSF)were infected with 107 L. chagasi promastigotes at 11th feeding week and euthanatized four-week post-infection. A diet high in simple carbohydrates and saturated fat was efficient in the induction of experimental obesity in mice, causing systemic changes. Obese mice were able to generate a high inflammatory response in response to challenge with L. chagasi, but showed increased susceptibility to infection. These results show that a hypercaloric macroenvironment can directly interfere with responses.