Estudo molecular de vírus associados à diarreia em crianças quilombolas do norte do Estado do Espírito Santo

Abstract

Diarrhea is a major public health problem and its consequences are aggravated by lack of medical care, diagnosis and treatment support, greatly exposing children to death. This study aimed to conduct a surveillance between 2007 and 2010 followed by detection and molecular phylogenetic analysis of viral agents found in the feces of children with (symptomatic) and without diarrhea (asymptomatic), under 12 years, and the susceptibility to norovirus infection according to blood group antigen (HBGA). A total of 574 fecal samples were obtained and searched for: (i) rotavirus (RV), by polyacrylamide gel electrophoresis and enzyme immunoassay (ii) norovirus (NoV), by RT-PCR of the B viral genome region and genotypes and genogroups determined by PCR amplification and sequencing, respectively, for the regions C and D; (iii) adenovirus (HAdV), by PCR and nested-PCR for the hexon gene, species and genotype analysed by restriction enzyme SalI and sequenced and; (iv) astrovirus (AstV), by real-time PCR for ORF2. HBGA phenotypes were determined by gel-spinning and FUT2 and FUT3 genes, analyzed by PCR and sequencing from blood and saliva, respectively, obtained from children infected with NoV and adults.