Comparação do efeito da triiodotironina no potencial condrogênico in vitro entre células tronco mesenquimais de ratas wistar

Abstract

Mesenchymal stem cells (MSC) are undifferentiated and multipotent, capable of chondrogenic differentiation and may be influenced by several factors, including hormonal. The objective was to compare the effect of triiodothyronine (T3) at different concentrations and times, on the chondrogenic potential of MSC in bone marrow (BMMSC) and adipose tissue (ASC) in young Wistar rats. Both MSCs were extracted and cultivated in vitro in chondrogenic medium without T3 (Controls) and with T3 (0.01; 1; 100 and 1000 nM) each, totaling 10 groups. After seven, 14 and 21 days, gene expression of Sox9, Collagen II (Col II) and Aggrecan (Acan) were evaluated using qRT-PCR and chondrogenic matrix formation, by histological staining Alcian blue (AB) - periodic acid of Schiff (PAS). In Sox9 it increased the expression of BM-MSC with T3 100 nM at 7 days and T3 1000 nM at 14 days, and increased the expression of ASC with T3 1 nM at 14 days; in Col II it increased the expression of BM-MSC with T3 0.01 nM at 7 days and increased the expression of ASC with T3 1000 nM at 14 days; in Acan increased the expression of BM-MSC with T3 1 nM at 14 days and increased the expression of ASC with T3 1 nM at 21 days. Regarding the synthesis of AB-PAS+ chondrogenic matrix, at seven days of differentiation, ASC were superior to BM-MSC in the control group and at concentrations of T3 0.01 nM, 1 nM and 100 nM, remaining similar between MSCs at 14 and 21 days. It is concluded that the chondrogenic potential with chondrogenic matrix synthesis of ASC is precociously superior in relation to BM-MSC at 7 days, both without the addition of T3 and with the addition of this hormone at concentrations of 0.01 nM, 1 nM and 100 nM.