O efeito da dieta rica em carboidrato refinado sobre a matriz extracelular hepática aplicada à bioengenharia tecidual

Abstract

The gap between the need for livers for transplantation and the availability of organs paved the way for new alternatives. Decellularization and recellularization of liver scaffold appear as a promising and alternative technique for liver transplantation. However, the liver is an organ that can be affected by pathologies that affect the structure and composition of the extracellular matrix (ECM) as a result high refined carbohydrate-containing diet (HC), which directly affect the liver. This paper discusses the effect of 12-week HC diet on liver ECM. The liver was decellularized and the histological and DNA analysis was used. Immunohistochemistry was also used to evaluate glycoproteins such as laminine and fibronectin. The ultrastructure of the ECM fibers was evaluated by scanning electron microscopy (SEM) and possible conformational changes of collagen were evaluated by FTIR. Chow and HC had the livers completely decellularized evaluated by histology and with levels below 50ng/mg of DNA. The HC group presented increased collagen in the liver, characteristic of NAFLD, loss of laminine and fibronectin, in ECM, as well as changes in the ultrastructure of the scaffold. Both HC and Chow groups presented in the FTIR analysis peaks of amide band I, characteristic of collagen, and reduction after deconvolution of the secondary alpha-helix structure. Thus, both the livers of animals treated with HC diet, carrier NAFLD of and the livers of animals in the Chow group showed efficiency in the decellularization process, however the HC group showed increased collagen and altered scaffold fiber mesh, loss of laminine and fibronectin and reduction of secondary collagen alpha-helix structure. The effects of these changes may guide future recellularization works.