Avaliação do efeito protetor do extrato hidroalcoólico de eugenia uniflora na lesão renal aguda experimental

Abstract

Acute kidney injury (AKI) is characterized by a rapid and potentially reversible decline in renal function; however, the current treatment of AKI is not specific and is associated with limited supportive care. Considering the need for new therapeutic approaches, we believe that the hydroalcoholic extract of leaves of Eugenia uniflora (pitanga) because of its antioxidant properties, may be beneficial for the treatment of RAI induced by renal ischemia / reperfusion (I / R). Hydroalcoholic extract from Eugenia uniflora leaves (200mg / kg, oral) or vehicle (0.9% saline) was administered to Wistar rats prior to the induction of bilateral renal ischemia (45 minutes). After 24 hours of reperfusion, clearances of inulin and paraminothiazurate were performed to determine the glomerular filtration rate (GFR), renal plasma flow (RPF), renal blood flow (RBF) and renal vascular resistance (RVR). The production of superoxide anion, hydrogen peroxide, peroxynitrite, hydroxyl radical, activity of oxidant enzymes and antioxidants and apoptosis in the kidneys were evaluated by flow cytometry. Evaluations of the expression of the antioxidant enzymes were performed by the Western blot technique. I / R resulted in reduced TFG, FPR and FSR and increased RVR, but these parameters were improved in rats receiving the extract. Treatment with Eugenia uniflora extract resulted in a decrease in oxidative stress and an increase in the activity and expression of antioxidant enzymes and a decrease in apoptosis in the kidneys of rats submitted to I / R. Our data showed that the hydroalcoholic extract of leaves of Eugenia uniflora may be able to prevent or attenuate the loss of renal function induced by ischemia reperfusion through the reduction of oxidative stress and apoptosis, thus providing valuable information that may subsidize the incorporation of herbal medicines into SUS.