Efeitos do tratamento com Sildenafil sobre as células de baço de camundongos ApoE -/-

Abstract

The atherosclerosis is a chronic inflammatory process that happens in large vases. This pathological process can reach several organs weakening its functions. How the spleen is an organ that can be reached by atherosclerosis, and because of its importance to the immune system and prevention of various diseases, the aim of this study was to evaluate the treatment of selective inhibitor of phosphodiesterase 5 (Sildenafil) on the cells of that organ, about level of ROS, DNA fragmentation and cell cycle. For such porpose, were used mice ApoE - / - , male and divided in two groups, treated for 3 weeks with Sildenafil 40 mg / kg / day (ApoEs) or only water (ApoEv). For the control group (C57), C57BL/6 lineage mice were used. After 3 weeks of treatment, when the animals were 12 weeks old, euthanasia was performed to remove blood from the right ventricular cavity and dosing cholesterol. Splenectomy was performed and isolation of spleen cells by maceration and lysis of red blood cells. The isolated cells were tested in FC to evaluate the level of ●O2- and H2O2, and the percentage of cells with fragmented DNA and the G0/G1 phase of the cell cycle. The results were expressed as Mean ± SE, using ANOVA 1 way followed by post hoc Fisher, p <0.05. Treatment with Sildenafil reduced levels as the ● O2 (ApoEs: 6,6±0,30 vs. ApoEv: 8,4±0,67 vs. C57: 6,1±0,29 x 102 u.a) as H2O2 (ApoEs: 3,3±0,30 vs. ApoEv: 4,5±0,41 vs. C57: 2,5±0,21 x 103 a.u), suggesting an antioxidant effect of sildenafil. It was also observed reduction of fragmented DNA percentage (ApoEs: 0,74±0,19% vs. ApoEv: 1,98±0,23% vs. C57: 0,61±0,09%), probably due to lower incidence of DNA damage caused by ROS. Reducing of the damage in the DNA, allows cells to make the cell cycle, leaving, therefore, the quiescent phase, as the result of the percentage of cells in G0 / G1 (After: 89.59 ± 2.1% * vs. APOEL: 96.13 ± 2.2% vs. C57: 91.28 ± 2.0%). The results demonstrate the protective effect of sildenafil DNA from spleen cells of ApoE - / - mice, probably by reducing elevated levels of ROS in these animals.