Avaliação do perfil renal em ratos (Rattus norvegicus) tratados com glicocorticoides

Abstract

The glucocorticoids are a group of drugs used for the treatment of various diseases and clinical signs for its anti-inflammatory and immunosuppressive action. Prolonged use may induce electrolyte imbalances due to fluid retention and laboratory abnormalities in the kidney. The objective of this study was to evaluate the enzymatic activity of gamma-glutamyltransferase (GGT), creatinine, protein and protein/creatinine ratio in urine, urea and creatinine in the serum to compare treatments with glucocorticoids in rats. For this, were collected blood samples and urine samples from 21 animals, Rattus norvegicus, Wistar lineage, without clinical or laboratory changes. The animals were divided into three groups of seven rats, group 1 (G1), group 2 (G2) and group 3 (G3). All animals in the G1 received 50,0 mg/kg of hydrocortisone succinate. The animals of group 2 received 2,0 mg/kg methylprednisolone and G3 animals received 1,0 mg/kg of dexamethasone, every 24 hours, intramuscularly, for 7 days. The experiment was divided into two moments, with the initial moment (M1) before using of drugs and moment 2 (M2) after the end of seven days. For the clinical and laboratory evaluation, samples of blood and urine of the animals were collected in two moments M1 and M2 to perform hematological, biochemical and urinalysis. The treatment with glucocorticoids caused an increase in serum creatinine in the treated groups. However, one can not say that there was renal injury in rats treated since the markers Pu/Cu, enzymatic activity of urinary gGT, urinalysis and serum urea remained unchanged. There is no difference between the groups of rats treated with glucocorticoids of short, medium and long term in immunosuppressive doses, intramuscularly, for a period of 7 days.