Avaliação dos parâmetros comportamentais e de estresse oxidativo em animais submetidos à separação maternal e ao consumo de etanol em binge durante a adolescência

Abstract

Early life stress can generate long-lasting effects on brain and behavior during adulthood. Furthermore, stressful experiences stimulate abusive consumption of ethanol, which induces neuronal damages and contributes to increases on parameters of oxidative stress on Central Nervous System. The aim of this research was to evaluate the effects of Maternal Separation (MS) and of intermittent ethanol binge drinking in adolescent rats on behavioral and oxidative stress parameters. Pregnant Wistar rats were used. After birth of litters, the animals were divided into control group (NS) or maternal separation group (MS). Maternal separation was performed from 2 to 15 postnatal day, 3 hours daily. At postnatal day 35, animals were divided again into 3 groups: animals which received intragastric saline (vehicle group) or ethanol in doses of 3.0 or 6.0 g/kg. Ethanol was administered once a day, in a regimen of two consecutive days interspersed by two days without ethanol, totalizing 10 doses for chronic binge treatments. Acute binge exposure happened at postnatal day 35 until 37 postnatal day.